Hi, I'm trying to run the program WinMDI 2.9, a free software to display and treat results obtained with flowcytometer but I received a message saying: this app can't run on your PC (the only requirement demanded by the software is to have Windows). I've had already tried the troubleshooting with no success. The program was developed to run with windows interfaces and I can use it in my work with a Win 7 but not in my laptop (Win 8). Can you help? ***Removing personal information for security purposes*** Castro ***Personal information deleted by the moderator. Please see the for more information on how you can protect your privacy.***.

Hi, Thank you for posting your query on Microsoft Community. As per the description, I understand that you are unable to run a program.

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I would like to know some information. • What troubleshooting steps have you tried?

Dec 20, 2010 - Appendix 2: Materials and Methods for Phagocytosis and Flow. Additional software programs include CellQuest, WinMDI, EXPO, Paint-A-Gate, FloJo and MFI. Some of these software programs are available as free downloads. Student-Generated Questions (10), 1, 7, 8, 9.8 (71%), 7.4 (29%), (0%).

I suggest you to perform clean boot and check, using this Microsoft Article because may be a third party application is causing this issue. NOTE: A clean boot is performed to start Windows by using a minimal set of drivers and startup programs. This helps eliminate software conflicts that occur when you install a program or an update or when you run a program in Windows. You may also troubleshoot or determine what conflict is causing the problem by performing a clean boot. You must log on to the computer as an administrator to be able to perform a clean boot. Visio stencils patch panel. Your computer may temporarily lose some functionality when you perform a clean boot. When you start the computer normally, the functionality returns.

However, you may receive the original error message, or experience the original behavior if the problem still exists. If the computer is connected to a network, network policy settings may prevent you from following these steps. Once you perform clean boot do refer to the section “ How to reset the computer to start normally after clean boot troubleshooting” to boot the computer in normal mode. I would suggest you to try installing the program in Windows 7 compatibility mode and check if it works for you.

Make older programs compatible with this version of Windows: Hope this information helps. Please let us know if you need any other assistance with Windows in future. We will be happy to assist you.

Phagocytosis is central to immunity however a rapid and standardized method is much needed for quantitative assessment of the phagocytic process. We describe a real‐time flow cytometric method to quantitate the phagocytosis of fluorescent latex beads by human monocytes in serum‐free conditions. Effects of buffer composition, temperature, pH, and bead surface on phagocytic rate are described. The innate phagocytic ability of human monocytes from single subjects measured by this method was relatively stable over many months although phagocytosis rate varied as much as two‐fold between individuals. Comparable results were obtained with a simplified method using several mL of whole blood which is suitable for routine clinical application. This method also allows two‐color flow cytometric measurement of cytosolic calcium levels during the phagocytic uptake of fluorescent beads.

© 2013 International Society for Advancement of Cytometry. I ntroduction Phagocytosis is a fundamental biological function of the immune system.

While phagocytosis of IgG opsonized particles is an important component of acquired immunity, phagocytosis of non‐opsonized particles plays a key role in innate immunity. Both require recognition of foreign particles by one or more phagocytic receptors on the surface of phagocytes, including Fc receptors, complement receptors, various integrins and scavenger receptors as well as the recently identified P2X7‐non‐muscle myosin heavy chain IIA complex which recognizes a range of non‐opsonized particles including latex beads, and live and dead bacteria. Many studies have shown that failed or defective phagocytic ability is linked to pathogenic states of various age‐related diseases,, autoimmune diseases as well as infectious diseases. However, there are few studies which have quantitated the phagocytic ability of native or transfected cells to assess possible defects in innate immunity.